Full Custom Design of the Interface for an in vitro Neural Biosensor：Abstract—This paper describes the interface design for an in
vitro neural biosensor which tends to combine biological
neural network and integrated circuits together in a microsystem
for intelligent bio-computation. As a simple case,
design and fabrication process for the 4×4 Microelectrode-
Array (MEA) that interfaces neural tissue with circuits is
presented in detail. The experimental results have primarily
demonstrated good performance of the MEA that was
fabricated in IC Manufacture Center for IME of Tsinghua
University. A tentative 256×256 MEA containing two
128×128 MEAs (one for stimulation while the other for
recording) for the final biosensor can hopefully arise from it
by the same token. Additionally, we also briefly present the
front-end design for the neural recording in this paper.