CROSS THE PROTEIN SOLUBILITY BARRIER WITH
STRATAGENE'S ARCTICEXPRESS? COMPETENT CELLS,
FEATURING COLD-ADAPTED TECHNOLOGY. LOWER GROWTH
TEMPERATURES (11-13°C) AND PROTEIN FOLDING CHAP-
ERONINS PROVIDED BY ANTARCTIC ISOLATE, Oleispira
antarctica, ALLOW THESE CELLS TO PROPERLY PROCESS
YOUR PREVIOUSLY INSOLUBLE PROTEINS WITHOUT FUSION
TAGS OR COMPLICATED IN VITRO REFOLDING METHODS.
Increase Protein Solubility
E. coli is often the first choice for recombinant protein
expression because it is fast, simple, and typically produces
a lot of protein. High protein yield, however, can be
accompanied by incorrect folding, resulting in inactive
protein aggregates known as inclusion bodies. Aggregated
protein can be easier to purify, but in vitro refolding requires
many processing steps and there is no guarantee that it will
yield a comparable amount of biologically active product. Our
ArcticExpress? Competent Cellsa
are optimized to facilitate
proper protein folding, resulting in increased production of
soluble, potentially active protein (Figure 1).?