以一株耐高滲透壓的酵母菌種作為敏感材料, 研制的的生物傳感器可采用穩(wěn)態(tài)呼吸速率法進(jìn)行BOD 快速測(cè)定。該方法與廣為采用的五天生化需氧量標(biāo)準(zhǔn)稀釋測(cè)定法有良好的相關(guān)性, 線性范圍為0～ 200mg?L , 可在15m in 內(nèi)完成一個(gè)樣品BOD 的測(cè)定。測(cè)定結(jié)果具有良好的重現(xiàn)性和準(zhǔn)確度, 標(biāo)準(zhǔn)曲線的線性相關(guān)系數(shù)達(dá)到019987。
關(guān)　鍵　詞:BOD; 生物傳感器; 酵母細(xì)胞
Abstract: A biochem ical oxygen demand (BOD) senso r, p repared from a st rain of o smo to lerant yeast, is calibrated by determ ining the steady2state rate of its response in various standard gluco se?g lutam ic acid BOD2 check so lut ions1 The steady2state rate varies linearly w ith the five2day BOD values from 0 to 200 mg?L 1 Typ ical response t imes of the BOD senso r w ere 5～ 15m in1 The results show good recurrence and specificity, and the co rrelat ion coefficient of the calibra2 t ion curve w ere at 0199871
Key words: BOD; bio senso r; yeast