利用自組裝單分子膜技術(shù)將巰己基修飾的單鏈DNA 固定在金電極表面,以電活性的Hoechst33258 為指示劑,考察了單鏈DNA 修飾電極(ssDNA/ Au 電極) 、雙鏈DNA 修飾電極(dsDNA/ Au 電極) 的指示劑氧化峰電流, 并利用其差值(Δip) 與互補(bǔ)DNA濃度成線性關(guān)系對(duì)待測DNA濃度進(jìn)行定量。因此,DNA 電化學(xué)傳感器可用于特定DNA 序列的識(shí)別和測定。
關(guān)鍵詞: 脫氧核糖核酸; 電化學(xué)傳感器; 修飾電極
Abstract : Thiol2derivatized ss2DNA is fixed on the surface of Au electrode using self2assemble film method. Hoechst 33258 is used as electro2active indicator. The peak current of indicator on ss2DNA modified electrode and ds2DNA modi2 fied electrode is observed respectively ,the difference of which has linearity with concentration of complementary DNA. So it is used for quantification of specific DNA. DNA electrochemical sensor can be used in the recognition and detection of specific DNA.
Key words : DNA; electrochemical sensor ; modified electrode